Jay L. Vivian, PhD
Research Associate Professor
Scientific Director, Transgenic and Gene Targeting Institutional Facility, KUMC
Children's Mercy Hospital
PhD, University of Texas-Houston, 1999
Postdoctoral, University of North Carolina at Chapel Hill, 2000-2004
My research uses embryonic and induced pluripotent stem cells as genetic and developmental models, tools for genetic engineering of the mouse, and as reagents for regenerative medicine. My group is interested in understanding the signaling pathways and genetic hierarchies that regulate gene expression, stem cell self-renewal, and differentiation in pluripotent stem cells. A major project seeks to understand the function of TGF-beta-related signaling pathways in stem cell self-renewal. These efforts have uncovered important and previously uncharacterized roles for the Nodal and BMP signaling pathways in the dynamic heterogeneity that exists in pluripotent cells.
My group makes substantial use of mouse embryonic stem cells for genetic engineering to generate transgenic and mutant mice, for the development of animal models of congenital diseases mapped by human genetics studies. I have a sustained interest in developing novel strategies to generate mutant and transgenic animals to support these studies. These efforts leverage our knowledge of both stem cell and developmental biology.
A major focus of my group also involves the development of pluripotent stem cells and genetically modified mice for modeling rare undiagnosed neurodegenerative disorders. We use patient-specific induced pluripotent stem cell models and genetic modification of the mouse via genome editing to study novel variants identified in these patients.
Another area of interest involves the development of cellular therapies for spinal cord injury using human pluripotent stem cells differentiated to specific neural sublineages. This work makes use of novel engineering strategies for introducing reporters into human iPS cells to monitor directed neural differentiation, and for use in high-throughput screens.
Renaud SJ, Chakraborty D, Mason CW, Rumi MA, Vivian JL, Soares MJ. (2015). OVO-like 1 regulates progenitor cell fate in human trophoblast development. Proc Natl Acad Sci USA. 112(45):E6175-84.
Lakatos D, Travis ED, Pierson, KE, Vivian, JL, Czirok, A. (2014). Autocrine FGF feedback can establish distinct states of Nanog expression in pluripotent stem cells: a computational analysis. BMC Computational Biology. 8(1), 112.
Rumi, MA, Dhajal, P., Kubota, K., Chakraborty, D., Lei, T., Larson, M.A., Wolfe M.W., Roby K.F., Vivian, J.L., Soares, M.J. (2014). Generation of Esr1 knockout rats using zinc finger nuclease-mediated genome editing. Endocrinology, 155(5):1991-9.
Burgess-Galvin, K.E., Travis, E.D., Pierson, K.E., and Vivian, J.L. (2013). TGF-beta-related signaling in embryonic stem cell maintenance: self-renewal as a dynamic and regulated equilibrium. Stem Cells 31, 48-58.
Home, P, Biswarup Saha, B., Ray, S., Debasree Dutta, D., Gunewardena, S., Yoo, B., Vivian, J.L., Larson, M., Petroff, M. , Gallagher, P.G., Schulz, V., White, K.L., Thaddeus G. Golos, T.G. , Behr, B., Paul, S. (2012). Altered Subcellular Localization of Transcription Factor TEAD4 Regulates First Mammalian Cell Lineage Commitment. PNAS 109, 7362-7.
Burgess-Galvin, K.E. and Vivian, J.L. (2011). Transforming Growth Factor-beta superfamily in mouse embryonic stem cell self-renewal. Vitamins and Hormones 87: Stem Cell Regulators. 87, 341-65.
Copeland JN, Feng Y, Neradugomma NK, Fields PE, Vivian JL. (2011). Notch signaling regulates remodeling and vessel diameter in the extraembryonic yolk sac. BMC Dev Biol. 11, 12.
Feng Y, Yang Y, Ortega MM, Copeland JN, Zhang M, Jacob JB, Fields TA, Vivian JL, Fields PE. (2010) Early mammalian erythropoiesis requires the Dot1L methyltransferase. Blood. 116, 4483-91.
Galvin KE, Travis ED, Yee D, Magnuson T, Vivian JL. (2010) Nodal signaling regulates the bone morphogenic protein pluripotency pathway in mouse embryonic stem cells. J Biol Chem. 285, 19747-56.
Dutta D, Ray S, Vivian JL, Paul S. (2008) Activation of the VEGFR1 chromatin domain: an angiogenic signal-ETS1/HIF-2alpha regulatory axis. J Biol Chem. 283, 25404-13.
Kirn-Safran CB, Oristian DS, Focht RJ, Parker SG, Vivian JL, Carson DD. (2007) Global growth deficiencies in mice lacking the ribosomal protein HIP/RPL29. Dev Dyn. 236, 447-60.